Bacteriophages and bacteriophagetyping of Campylbacter Jejuni and Campylobacter Coli..

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A bacteriophage typing system for Campylobacter jejuni and Campylobacter coli was developed with phages isolated from poultry feces. Data for phage selection were generated from a set of isolates of C.

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jejuni and C. coli from humans in by: Developmentofa Bacteriophage Typing Systemfor Campylobacter jejuni and Campylobactercoli BARBARAA. GRAJEWSKI,*JOHNW. KUSEK,tANDHENRYM.

Details Bacteriophages and bacteriophagetyping of Campylbacter Jejuni and Campylobacter Coli.. EPUB

GELFAND Epidemiology-Biometry Program, SchoolofPublic Health, University ofIllinois, Chicago, Illinois Received 17 December/Accepted 14 March Cited by: A bacteriophage typing system for Campylobacter jejuni and Bacteriophages and bacteriophagetyping of Campylbacter Jejuni and Campylobacter Coli.

book coli was developed with phages isolated from poultry feces. Data for phage selection were generated from a set of isolates of C. jejuni and C. coli from humans in Illinois. Selection of 14 phages from the 47 phages available was assisted by determination of the Sneath-Jaccard similarity coefficients and subsequent Cited by: Campylobacter bacteriophages and bacteriophage therapy P.L.

Connerton, A.R. Timms and I.F. Connerton principally Campylobacter jejuni and Campylobacter coli that account for the majority of cases of bacterial gastro- example their use in bacteriophage-typing schemes or for the rapid identification of bacteria, the recent focus of Cited by: The extended phage-typing scheme described for Campylobacter jejuni and Campylobacter coli has established 46 different phage types using 19 typing phages.

Altogether campylobacter isolates, C. jejuni and 82 C. coli, isolated from human and non-human sources received from 17 different countries were l, 806% of the total isolates were by: Campylobacter jejuni is a leading cause of foodborne illness globally.

In this study, a spray drying and packaging process was developed to produce a thermally-stable dry powder containing bacteriophages that retains biological activity against C.

jejuni after long distance shipping at ambient temperature. Spray drying using a twin-fluid atomizer resulted in significantly less (p.

Campylobacter species have a chemoorganotrophic metabolism, and energy is derived from amino acids or tricarboxylic acid cycle intermediates due to their inability to oxidize or ferment carbohydrates.

The majority of Campylobacter spp. reduce nitrate and nitrite. Campylobacter jejuni contains two flagellin genes, flaA and flaB; the wild-type bacterium expresses flaA only, but flaB can be. The extended phage-typing scheme described for Campylobacter jejuni and Campylobacter coli has established 46 different phage types using 19 typing phages.

Altogether campylobacter isolates, C. jejuni and 82 C. coli, isolated from human and non-human sources received from 17 different countries were phage-typed. Med. Microbiol. - Vol. 47 (), 0 The Pathological Society of Great Britain and Ireland E PI D E M I0 LOG I CAL TY P I NG Characterisation of 16 Campylobacter jejuni and C.

coli. The kDa protein PEB4, a major antigen of Campylobacter jejuni, is present in all C. jejuni strains tested and elicits an antibody response in infected patients.

By screening a λgt11 library of chromosomal DNA fragments of C. jejuni strain 81– in Escherichia coli Y cells with antibody raised against purified PEB4, a recombinant phage with a 2-kb insert expressing an immunoreactive.

INTRODUCTION. Campylobacteriosis is a common food-borne zoonosis worldwide. Init was the most frequent food-borne bacterial enteritis in Germany, with more t reported cases (), and inthere werecases in the European Union (EU) ().The thermotolerant Campylobacter species C.

jejuni and C. coli are the most frequently isolated agents, and symptoms in humans range. An important breakthrough was the publication of the sequences and genomic analysis of two Campylobacter group II bacteriophages with broad lytic activity against both C. jejuni and C.

coli isolates (Timms et al. The genomes of the two bacteriophages studied were extremely similar at the nucleotide level despite the fact that they were isolated from different places. Livestock such as poultry is consumed as food worldwide and it constitutes one of the main protein sources for diners, as well as an important source of revenue generation for farmers.

Poultry meat production chain identifies a significant role of the farm to folk. Most often, the systems used in poultry production can result in a higher prevalence of Campylobacter, Salmonella, and Escherichia.

Grajewski BA, Kusek JW, Gelfand HM () Development of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli. J Clin Microbiol 13–18 [. Cattle are an established reservoir of the foodborne bacterial pathogen Campylobacter jejuni.

Our six-month study aimed to evaluate sources and pathways governing long-term presence of C. jejuni in a pasture-based dairy herd. jejuni was detected in all sample types (soil, pasture, stock drinking water, bird, rodents and cow faeces).

It was persistently detected from cow (54%; 49/90 samples. Domain: Bacteria - Like many other types of bacteria, the genus Campylobacter belongs to the domain Bacteria that consists of prokaryotic microorganisms. Phylum: Campylobacter belongs to the phylum Proteobacteria.

This is a major phylum composed of Gram-negative bacteria and includes such bacteria as books refer to this as the epsilon-subdivision. Sixteen Campylobacter-specific phages are used for the epidemiological typing of C.

jejuni and C. coli in the United Kingdom. The original phage-typing scheme was described in the United States in using 14 phages isolated from poultry feces (22) and was later extended to.

Campylobacter jejuni and C. coli collectively account for the majority of acute bacterial enteritis cases reported in the United Kingdom and most of the developed world ().More than 80% of birds in the United Kingdom harbor these organisms as a part of their normal intestinal flora (9, 26).Large numbers of these bacteria are disseminated upon abattoir processing and can be readily isolated.

Campylobacter-specific bacteriophages have been isolated from various sources including pig manure, ab-attoir effluents, sewage, and broiler chickens (2, 15, 22, 35). Some of these bacteriophages have been characterized to form the basis of the United Kingdom phage typing scheme (13, 34).

Bacteriophages have been applied as a decontamination. Abstract. Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces.

The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4. Abstract. Campylobacteriosis is a frequently diagnosed disease in humans.

Most infections are considered food-borne and are caused by Campylobacter jejuni and C. animal reservoirs of these Campylobacter, and the sources and routes of transmission, are described and discussed. Most warm-blooded animals can be colonized by Campylobacter, but avians, and in particular poultry, are.

The most important Campylobacter species in human gastroenteritis is Campylobacter jejuni, which accounts for 90 to 95% of all campylobacteriosis cases reported. The majority of the remaining cases are caused by Campylobacter coli, but the importance of C.

coli as an enteric pathogen varies between regions. Campylobacter jejuni gastroenteritis is the most frequent organism associated with acute infectious diarrhea worldwide.

The clinical presentation involves fever, diarrhea, rigors, and myalgias. Other extraintestinal symptoms that have been described involve delirium and other neurological complications, and the most well-known is Guillain-Barré, where there is cross-reactivity between the.

Abstract. A bacteriophage typing system for Campylobacter jejuni and Campylobacter coli was developed with phages isolated from poultry feces. Data for phage selection were generated from a set of isolates of C. jejuni and C.

Description Bacteriophages and bacteriophagetyping of Campylbacter Jejuni and Campylobacter Coli.. FB2

coli from humans in Illinois. Bacteriophage therapy can potentially reduce Campylobacter jejuni numbers in livestock, but it requires a detailed understanding of phage-host interactions. jejuni strains readily infected by certain phages are designated as phage-propagating strains.

Here, we report the complete genome sequences of three such strains, NCTCNCTCand NCTC Lytic Campylobacter phages, which can be used to combat this pathogen in animals and on food products, have been studied for more than 30 years. Though, due to some peculiarities of the phages, which hampered their isolation and particularly their molecular analysis for a long time, progress in this research field was rather slow.

Meanwhile, the situation has changed and much more is known. Prevalence estimates vary from 71% C. jejuni/C.

coli in broilers, 45% C. jejuni in dogs, 36% C. jejuni in beef cattle, and 42% C. coli in pigs. Presence of Campylobacter in these animals is usually asymptomatic, although in cattle and sheep C. jejuni has been reported to cause sporadic abortions.

This chapter discusses efforts to exploit Campylobacter -specific bacteriophages to reduce the numbers of C. jejuni and C. coli colonizing poultry and contaminating poultry meat products.

All the phages reported by investigators in two studies had icosahedral heads and long contractile tails that were classified as members of the Myoviridae. jejuni L26 or 49 as a host showed different lytic spectra.

Of the 26 phages, phage isolate PHC10 showed the broadest lytic spectrum, lysing 31 of 46( %) C. Campylobacter jejuni is a major cause of acute bacterial enteritis worldwide.

Domestic poultry are a reservoir for campylobacters and undercooked poultry meat, in particular chicken, is by implication an etiological source of human infections ().One potential mechanism to control Campylobacter is the use of host-specific bacteriophage (3, 8).Previous studies have shown that there is a high.

Campylobacter is a gram-negative, microaerophilic genus of bacteria of the family are more than 20 species of Campylobacter, not all of which cause human imately 90% of human Campylobacter illness is caused by one species, Campylobacter jejuni.

Less common species, such as C. coli, C. upsaliensis, C. fetus, and C. lari, can also infect .Abstract. Campylobacter subspecies represent a highly frequent cause of foodborne gastrointestinal (GI) illnesses in humans worldwide.

The incidence and prevalence of campylobacteriosis due to infection with Campylobacter jejuni has increased over the past decades, both in developed and developing countries. The clinical outcome of C. jejuni infection ranges from mild to severe diarrheal.

Diagnosis Campylobacter infection is diagnosed when a laboratory test detects Campylobacter bacteria in stool (poop), body tissue, or fluids. The test could be a culture that isolates the bacteria or a rapid diagnostic test that detects genetic material of the bacteria.

Treatment Most people recover from Campylobacter infection without antibiotic treatment.